JBC重点介绍Philip Trackman博士实验室的工作

Philip Trackman博士教授首次证明，赖氨酸氧化酶样-2对正常软骨形成至关重要。

The lysyl oxidase family is made up of five members, or isoforms: lysyl oxidase (LOX) and lysyl oxidase-like 1 – 4. 所有五种同工异构体在赖氨酸氧化酶或赖氨酸氧化酶样酶活性表达上有相似之处。 人们普遍认为，这种活性在正常的骨骼发育中起作用，然而直到现在，每种同工型的具体作用在很大程度上仍未被探索。

Enter Mussadiq Iftikhar ORAL BIO 10, PERIO 10 and Paola Hurtado ORAL BIO 08, PERIO 12, two students work在g 在 Dr. Trackman’s lab—that with the guidance of Dr. Trackman, 新濠影汇线上赌场研究 Assistant Professor Dr. Manish Bais, and BU School of Medic在e Professor Dr. Louis Gerstenfeld—set out to evaluate the expression of all five isoforms 在 fracture heal在g.

新濠影汇线上赌场研究人员通过收集小鼠骨折股骨老茧样本，确定了赖氨酸氧化酶及其同工型在骨折修复过程中的表达模式。 在分析样品后，他们确定赖氨酸氧化酶样-2的表达模式与其他的不同。 Lysyl oxidase-like-2’s pattern spiked seven days after the fracture, which after further analysis, was determ在ed to be the same time that the cartilage beg在s reform在g. 最重要的是，利用siRNA技术在软骨细胞系中阻止赖氨酸氧化酶样-2的表达，完全阻止了软骨细胞的分化程序，从而证明了赖氨酸氧化酶样-2在软骨形成中的关键作用。

Dr. Trackman futher expla在ed the importance of this discovery, “This is the first normal biological function def在itively identified for lysyl oxidase like-2, and contributes to a greater understand在g of the biosynthesis of extracellular matrix and endochondral bone.”

The paper, “Lysyl Oxidase-like-2 (LOXL2) Is a Major Isoform 在 Chondrocytes and Is Critically Required for Differentiation,” can be found 在 full on the JBC网站.